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Genetics & Biotechnology vocabulary · Sequencing and Genomics

Sanger sequencing

Meaning in genetics & biotechnology, with examples

nounCommon term

In genetics & biotechnology, Sanger sequencing means: The classic DNA sequencing method that reads one fragment at a time, up to about 1,000 bases, with very high accuracy. It is named after Frederick Sanger.

In Turkish: Sanger dizileme

How it is used

Still used daily to check plasmids, confirm CRISPR edits and validate variants found by NGS. Results come as a chromatogram, also called a trace. People 'send samples for Sanger' and look for 'clean peaks'. Two overlapping peaks at one position can mean a heterozygous variant.

Example sentences

  1. We confirmed every NGS finding by Sanger sequencing before reporting.

  2. Sanger sequencing of the plasmid shows that the insert is in the right orientation.

Common collocations

  • Sanger sequencing confirmation
  • send for Sanger sequencing
  • Sanger trace
  • chromatogram
  • double peak
  • sequencingAny method that works out the exact order of bases (A, T, G and C) in a piece of DNA or RNA.
  • primerA short, single-stranded piece of DNA, usually 18 to 25 bases long, that binds to a matching sequence and gives DNA polymerase a starting point for copying.
  • next-generation sequencingModern technologies that read millions or billions of DNA fragments in parallel, making it fast and cheap to sequence whole genomes, exomes or many genes at once.
  • heterozygousHaving two different alleles of a particular gene, one inherited from each parent.

More terms: Sequencing and Genomics

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