Restriction enzyme

How it is used

Used in classic cloning, plasmid checks and some genotyping tests. Researchers 'digest' DNA with restriction enzymes and run the 'digest' on a gel. Enzymes leave either sticky ends or blunt ends. They are also called restriction endonucleases.

Example sentences

1. We cut the plasmid with two restriction enzymes to check the insert.

2. The variant destroys a restriction enzyme site, so we can genotype it with a simple digest.

Common collocations

• restriction enzyme digest
• restriction site
• cut with a restriction enzyme
• sticky ends
• double digest

Related terms

• plasmidA small, circular piece of DNA found naturally in bacteria and used in the lab as a tool to carry and copy genes inside cells.
• cloneTo make copies of a DNA fragment by inserting it into a vector and growing it in cells; also, to make a genetically identical copy of a cell or organism.
• vectorA DNA molecule, such as a plasmid or a modified virus, used to carry a gene into a cell, where it can be copied or expressed.
• gel electrophoresisA method that separates DNA, RNA or protein fragments by size by pulling them through a gel with an electric current; smaller fragments move faster.

More terms: Genetic Engineering and CRISPR

• CRISPR
• genetic engineering
• GMO
• guide RNA
• gene editing
• knockout
• transfection
• off-target effect